Staff from CVS practices trekked 25 miles along Hadrian’s Wall to raise funds for the charity Support Dogs.
The adventurers have raised more than £8,000, exceeding their target by more than £5,000.
Three teams primarily of vets, nurses and support staff took part in the challenge.
They started at the ancient Roman fort of Brocolitia, near Carrawburgh in Northumberland and finished at Lanercost Priory in Cumbria just over nine hours later.
CVS marketing and commercial director Barry Brackner said: “Many of us were a little sore and nursing blisters for a few days afterwards but we all thoroughly enjoyed the challenge and were grateful for dry, mild weather.
“We are delighted to have raised so much money for Support Dogs. It will be added to the total we have raised from fund-raising activities across the business, including the highly competitive bake-off underway at our support office. The final total will be announced at CVS Conference in November.”
Support Dogs is a national charity dedicated to increasing independence and quality of life for people with medical conditions. It trains autism assistance dogs, disability assistance dogs and seizure alert dogs.
Chief executive Rita Howson said: “What a fantastic result from the CVS teams.
“Support Dogs has a life-changing impact on all those we help and, as a result, we are experiencing an overwhelming demand for our work with a plea for help coming in every 10 hours.
“This contribution will be really help us, so we are delighted to be CVS’ charity of the year.”